Engineering human hepato-biliary-pancreatic organoids from pluripotent stem cells.

ABSTRACT: Human organoids are emerging as a valuable resource to investigate human organ development and disease. The applicability of human organoids has been limited, partly due to the oversimplified architecture of the current technology, which generates single-tissue organoids that lack inter-organ structural connections. Thus, engineering organoid systems that incorporate connectivity between neighboring organs is a critical unmet challenge in an evolving organoid field. Here, we describe a protocol for the continuous patterning of hepatic, biliary and pancreatic (HBP) structures from a 3D culture of human pluripotent stem cells (PSCs). After differentiating PSCs into anterior and posterior gut spheroids, the two spheroids are fused together in one well. Subsequently, self-patterning of multi-o

SUBMITTER: Koike H 

PROVIDER: S-EPMC8212777 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature