Nonameric structures of the cytoplasmic domain of FlhA and SctV in the context of the full-length protein.

ABSTRACT: Type three secretion is the mechanism of protein secretion found in bacterial flagella and injectisomes. At its centre is the export apparatus (EA), a complex of five membrane proteins through which secretion substrates pass the inner membrane. While the complex formed by four of the EA proteins has been well characterised structurally, little is known about the structure of the membrane domain of the largest subunit, FlhA in flagella, SctV in injectisomes. Furthermore, the biologically relevant nonameric assembly of FlhA/SctV has been infrequently observed and differences in conformation of the cytoplasmic portion of FlhA/SctV between open and closed states have been suggested to reflect secretion system specific differences. FlhA has been shown to bind to chaperone-substrate complexes in

SUBMITTER: Kuhlen L 

PROVIDER: S-EPMC8213127 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature