Improved preservation of ovarian tissue morphology that is compatible with antigen detection using a fixative mixture of formalin and acetic acid.
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ABSTRACT:
SUBMITTER: Adeniran BV
PROVIDER: S-EPMC8213453 | biostudies-literature | 2021 Jun
REPOSITORIES: biostudies-literature
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Improved preservation of ovarian tissue morphology that is compatible with antigen detection using a fixative mixture of formalin and acetic acid.
Adeniran B V BV Bjarkadottir B D BD Appeltant R R Lane S S Williams S A SA
Human reproduction (Oxford, England) 20210601 7
<h4>Study question</h4>Can ovarian tissue morphology be better preserved whilst enabling histological molecular analyses following fixation with a novel fixative, neutral buffered formalin (NBF) with 5% acetic acid (referred to hereafter as Form-Acetic)?<h4>Summary answer</h4>Fixation with Form-Acetic improved ovarian tissue histology compared to NBF in multiple species while still enabling histological molecular analyses.<h4>What is known already</h4>NBF fixation results in tissue shrinkage in ...[more]
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