Hsp-90 and unc-45 depletion induce characteristic transcriptional signatures in coexpression cliques of C. elegans.

ABSTRACT: Nematode development is characterized by progression through several larval stages. Thousands of genes were found in large scale RNAi-experiments to block this development at certain steps, two of which target the molecular chaperone HSP-90 and its cofactor UNC-45. Aiming to define the cause of arrest, we here investigate the status of nematodes after treatment with RNAi against hsp-90 and unc-45 by employing an in-depth transcriptional analysis of the arrested larvae. To identify misregulated transcriptional units, we calculate and validate genome-wide coexpression cliques covering the entire nematode genome. We define 307 coexpression cliques and more than half of these can be related to organismal functions by GO-term enrichment, phenotype enrichment or tissue enrichment analysis. Impor

SUBMITTER: Schmauder L 

PROVIDER: S-EPMC8213770 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature