Measurement of lipogenic flux by deuterium resolved mass spectrometry.

ABSTRACT: De novo lipogenesis (DNL) is disrupted in a wide range of human disease. Thus, quantification of DNL may provide insight into mechanisms and guide interventions if it can be performed rapidly and noninvasively. DNL flux is commonly measured by ²H incorporation into fatty acids following deuterated water (²H₂O) administration. However, the sensitivity of this approach is limited by the natural abundance of ¹³C, which masks detection of ²H by mass spectrometry. Here we report that high-resolution Orbitrap gas-chromatography mass-spectrometry resolves ²H and ¹³C fatty acid mass isotopomers, allowing DNL to be quantified using lower ²H₂O doses and shorter experimental periods than previously poss

SUBMITTER: Fu X 

PROVIDER: S-EPMC8213799 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature