ON-bipolar cell gene expression during retinal degeneration: Implications for optogenetic visual restoration.

ABSTRACT:

Purpose

Retinal bipolar cells survive even in the later stages of inherited retinal degenerations (IRDs) and so are attractive targets for optogenetic approaches to vision restoration. However, it is not known to what extent the remodelling that these cells undergo during degeneration affects their function. Specifically, it is unclear if they are free from metabolic stress, receptive to adeno-associated viral vectors, suitable for opsin-based optogenetic tools and able to propagate signals by releasing neurotransmitter.

Methods

Fluorescence activated cell sorting (FACS) was performed to isolate labelled bipolar cells from dissociated retinae of litter-mates with or without the IRD mutation Pde6b^rd1/rd1 selectively expressing an enhanced yellow fluorescent protein

SUBMITTER: Gilhooley MJ 

PROVIDER: S-EPMC8214074 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature