Combining QTL-seq and linkage mapping to fine map a candidate gene in qCTS6 for cold tolerance at the seedling stage in rice.

ABSTRACT:

Background

Cold stress caused by low temperatures is an important factor restricting rice production. Identification of cold-tolerance genes that can stably express in cold environments is crucial for molecular rice breeding.

Results

In this study, we employed high-throughput quantitative trait locus sequencing (QTL-seq) analyses in a 460-individual F_2:3 mapping population to identify major QTL genomic regions governing cold tolerance at the seedling stage in rice. A novel major QTL (qCTS6) controlling the survival rate (SR) under low-temperature conditions of 9°C/10 days was mapped on the 2.60-Mb interval on chromosome 6. Twenty-seven single-nucleotide polymorphism (SNP) markers were designed for the qCST6 region based on re-sequencing data, and local QTL mapping

SUBMITTER: Yang L 

PROVIDER: S-EPMC8214256 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature