Homoeolog Inference Methods Requiring Bidirectional Best Hits or Synteny Miss Many Pairs.

ABSTRACT: Homoeologs are pairs of genes or chromosomes in the same species that originated by speciation and were brought back together in the same genome by allopolyploidization. Bioinformatic methods for accurate homoeology inference are crucial for studying the evolutionary consequences of polyploidization, and homoeology is typically inferred on the basis of bidirectional best hit (BBH) and/or positional conservation (synteny). However, these methods neglect the fact that genes can duplicate and move, both prior to and after the allopolyploidization event. These duplications and movements can result in many-to-many and/or nonsyntenic homoeologs-which thus remain undetected and unstudied. Here, using the allotetraploid upland cotton (Gossypium hirsutum) as a case study, we show that conventional

SUBMITTER: Glover N 

PROVIDER: S-EPMC8214411 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature