Aligned human cardiac syncytium for in vitro analysis of electrical, structural, and mechanical readouts.

ABSTRACT: Human pluripotent stem cell-derived cardiomyocytes (hPSC-CMs) have emerged as an exciting new tool for cardiac research and can serve as a preclinical platform for drug development and disease modeling studies. However, these aspirations are limited by current culture methods in which hPSC-CMs resemble fetal human cardiomyocytes in terms of structure and function. Herein we provide a novel in vitro platform that includes patterned extracellular matrix with physiological substrate stiffness and is amenable to both mechanical and electrical analysis. Micropatterned lanes promote the cellular and myofibril alignment of hPSC-CMs while the addition of micropatterned bridges enable formation of a functional cardiac syncytium that beats synchronously over a large two-dimensional area. We investig

SUBMITTER: Napiwocki BN 

PROVIDER: S-EPMC8214444 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature