Genome-wide imaging screen uncovers molecular determinants of arsenite-induced protein aggregation and toxicity.

ABSTRACT: The toxic metalloid arsenic causes widespread misfolding and aggregation of cellular proteins. How these protein aggregates are formed in vivo, the mechanisms by which they affect cells and how cells prevent their accumulation is not fully understood. To find components involved in these processes, we performed a genome-wide imaging screen and identified Saccharomyces cerevisiae deletion mutants with either enhanced or reduced protein aggregation levels during arsenite exposure. We show that many of the identified factors are crucial to safeguard protein homeostasis (proteostasis) and to protect cells against arsenite toxicity. The hits were enriched for various functions including protein biosynthesis and transcription, and dedicated follow-up experiments highlight the importance of accur

SUBMITTER: Andersson S 

PROVIDER: S-EPMC8214759 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature