Asymmetric dynamics of dimeric SARS-CoV-2 and SARS-CoV main proteases in an apo form: Molecular dynamics study on fluctuations of active site, catalytic dyad, and hydration water.

ABSTRACT: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has been widely spread around the world. It is necessary to examine the viral proteins that play a notorious role in the invasion of our body. The main protease (3CL^pro) facilitates the maturation of the coronavirus. It is thought that the dimerization of 3CL^pro leads to its catalytic activity; the detailed mechanism has, however, not been suggested. Furthermore, the structural differences between the predecessor SARS-CoV 3CL^pro and SARS-CoV-2 3CL^pro have not been fully understood. Here, we show the structural and dynamical differences between the two main proteases, and demonstrate the relationship between the dimerization and the activity via atomistic molecular dynamics simulations.

SUBMITTER: Iida S 

PROVIDER: S-EPMC8214910 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature