Arabidopsis thimet oligopeptidases are redox-sensitive enzymes active in the local and systemic plant immune response.

ABSTRACT: Upon pathogen infection, receptors in plants will activate a localized immune response, the effector-triggered immunity (ETI), and a systemic immune response, the systemic acquired response (SAR). Infection also induces oscillations in the redox environment of plant cells, triggering response mechanisms involving sensitive cysteine residues that subsequently alter protein function. Arabidopsis thaliana thimet oligopeptidases TOP1 and TOP2 are required for plant defense against pathogens and the oxidative stress response. Herein, we evaluated the biochemical attributes of TOP isoforms to determine their redox sensitivity using ex vivo Escherichia coli cultures and recombinant proteins. Moreover, we explored the link between their redox regulation and plant immunity in wild-type and mutant A

SUBMITTER: Al-Mohanna T 

PROVIDER: S-EPMC8215294 | biostudies-literature | 2021 Jan-Jun

REPOSITORIES: biostudies-literature