Integrating Transcriptome-Wide Association Study and mRNA Expression Profiling Identifies Novel Genes Associated With Osteonecrosis of the Femoral Head.

ABSTRACT:

Objective

This study aims to identify novel candidate genes associated with osteonecrosis of the femoral head (ONFH).

Methods

A transcriptome-wide association study (TWAS) was performed by integrating the genome-wide association study dataset of osteonecrosis (ON) in the UK Biobank with pre-computed mRNA expression reference weights of muscle skeleton (MS) and blood. The ON-associated genes identified by TWAS were further subjected to gene ontology (GO) analysis by the DAVID tool. Finally, a trans-omics comparative analysis of TWAS and genome-wide mRNA expression profiling was conducted to identify the common genes and the GO terms shared by both DNA-level TWAS and mRNA-level expression profile for ONFH.

Results

TWAS totally identified 564 genes that were with P

SUBMITTER: Ma M 

PROVIDER: S-EPMC8215574 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature