Integrated multi-omics analyses on patient-derived CRC organoids highlight altered molecular pathways in colorectal cancer progression involving PTEN.

ABSTRACT:

Background

Colorectal cancer (CRC) represents the fourth leading cause of cancer-related deaths. The heterogeneity of CRC identity limits the usage of cell lines to study this type of tumor because of the limited representation of multiple features of the original malignancy. Patient-derived colon organoids (PDCOs) are a promising 3D-cell model to study tumor identity for personalized medicine, although this approach still lacks detailed characterization regarding molecular stability during culturing conditions. Correlation analysis that considers genomic, transcriptomic, and proteomic data, as well as thawing, timing, and culturing conditions, is missing.

Methods

Through integrated multi-omics strategies, we characterized PDCOs under different growing and timing conditions,

SUBMITTER: Codrich M 

PROVIDER: S-EPMC8215814 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature