Single-Cell RNA-Sequencing Reveals the Breadth of Osteoblast Heterogeneity.

ABSTRACT: The current paradigm of osteoblast fate is that the majority undergo apoptosis, while some further differentiate into osteocytes and others flatten and cover bone surfaces as bone lining cells. Osteoblasts have been described to exhibit heterogeneous expression of a variety of osteoblast markers at both transcriptional and protein levels. To explore further this heterogeneity and its biological significance, Venus-positive (Venus⁺) cells expressing the fluorescent protein Venus under the control of the 2.3-kb Col1a1 promoter were isolated from newborn mouse calvariae and subjected to single-cell RNA sequencing. Functional annotation of the genes expressed in 272 Venus⁺ single cells indicated that Venus⁺ cells are osteoblasts that can be categorized i

SUBMITTER: Yoshioka H 

PROVIDER: S-EPMC8216137 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature