G-quadruplex-forming aptamer enhances the peroxidase activity of myoglobin against luminol.

ABSTRACT: Aptamers can control the biological functions of enzymes, thereby facilitating the development of novel biosensors. While aptamers that inhibit catalytic reactions of enzymes were found and used as signal transducers to sense target molecules in biosensors, no aptamers that amplify enzymatic activity have been identified. In this study, we report G-quadruplex (G4)-forming DNA aptamers that upregulate the peroxidase activity in myoglobin specifically for luminol. Using in vitro selection, one G4-forming aptamer that enhanced chemiluminescence from luminol by myoglobin's peroxidase activity was discovered. Through our strategy-in silico maturation, which is a genetic algorithm-aided sequence manipulation method, the enhancing activity of the aptamer was improved by introducing mutations to t

SUBMITTER: Tsukakoshi K 

PROVIDER: S-EPMC8216272 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature