AniFOUND: analysing the associated proteome and genomic landscape of the repaired nascent non-replicative chromatin.

ABSTRACT: Specific capture of chromatin fractions with distinct and well-defined features has emerged as both challenging and a key strategy towards a comprehensive understanding of genome biology. In this context, we developed aniFOUND (accelerated native isolation of factors on unscheduled nascent DNA), an antibody-free method, which can label, capture, map and characterise nascent chromatin fragments that are synthesized in response to specific cues outside S-phase. We used the 'unscheduled' DNA synthesis (UDS) that takes place during the repair of UV-induced DNA lesions and coupled the captured chromatin to high-throughput analytical technologies. By mass-spectrometry we identified several factors with no previously known role in UVC-DNA damage response (DDR) as well as known DDR proteins. We ex

SUBMITTER: Stefos GC 

PROVIDER: S-EPMC8216468 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature