Recruitment of release sites underlies chemical presynaptic potentiation at hippocampal mossy fiber boutons.

ABSTRACT: Synaptic plasticity is a cellular model for learning and memory. However, the expression mechanisms underlying presynaptic forms of plasticity are not well understood. Here, we investigate functional and structural correlates of presynaptic potentiation at large hippocampal mossy fiber boutons induced by the adenylyl cyclase activator forskolin. We performed 2-photon imaging of the genetically encoded glutamate sensor iGluu that revealed an increase in the surface area used for glutamate release at potentiated terminals. Time-gated stimulated emission depletion microscopy revealed no change in the coupling distance between P/Q-type calcium channels and release sites mapped by Munc13-1 cluster position. Finally, by high-pressure freezing and transmission electron microscopy analysis, we fou

SUBMITTER: Orlando M 

PROVIDER: S-EPMC8216508 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature