An Intravital Microscopy Toolbox to Study Mammary Gland Dynamics from Cellular Level to Organ Scale.

ABSTRACT: The architecture of the mouse mammary gland is highly dynamic and constantly remodeled during pubertal development and estrous cycle-driven sprouting and regression of alveolar side branches. During each of these developmental stages, turnover is driven by distinct subsets of mammary epithelial cells. Extensive previous research has shed light on the unique morphological and cell biological characteristics of each stage. However, technological shortcomings failed to capture the dynamics and single-cell contributions to mammary remodeling. Here, we developed in vivo imaging strategies to follow the same mammary ducts over time and quantify the dynamics of mammary gland growth and remodeling from single-cell level to organ scale. Using a combination of intravital microscopy and genetic repor

SUBMITTER: Messal HA 

PROVIDER: S-EPMC8217050 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature