Differential effects of dietary cholesterol and triglycerides on the lipid homeostasis in Meibomian glands.

ABSTRACT: Exocrine Meibomian glands (MG) play a central role in the ocular surface physiology by producing meibum - a lipid secretion composed of cholesteryl esters (CE), cholesterol (Chl), triacylgycerols (TAG), waxes and other types of lipids. MG were previously shown to synthesize Meibomian lipids (ML) in situ via a complex array of reactions termed meibogenesis. However, questions remain about the role of dietary lipids in meibogenesis. To establish if dietary Chl (DC) and TAG (DT) can participate in meibogenesis, we studied mice whose diet was supplemented with trace amounts of deuterated Chl (²H-Chl) and ¹³C-labeled triolein (¹³C-TO), and the products of their biosynthetic transformations were analyzed using LC/MS. We demonstrated that ²H-Chl, but no

SUBMITTER: Butovich IA 

PROVIDER: S-EPMC8217181 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature