Modeling the mature CNS: A predictive screening platform for neurodegenerative disease drug discovery.

ABSTRACT:

Background

Mature primary neuronal cultures are an important model of the nervous system, but limited scalability has been a major challenge in their use for drug discovery of neurodegenerative diseases. This work describes a method for improving scalability through the use of larger format microtiter plates while preserving culture quality.

New method

Here we describe a method and quality control procedures for growing embryonic day 18 rat hippocampal/cortical neuronal cultures in 384-well microtiter plates for three weeks in vitro.

Results

We use these cultures in two assays measuring intracellular lipid vesicle trafficking and synapse density for routine screening of small molecule libraries. Together this culture system and screening platform have successfully ide

SUBMITTER: LaBarbera KM 

PROVIDER: S-EPMC8217273 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature