A 3D cell culture approach for studying neuroinflammation.

ABSTRACT:

Background

Neurodegenerative diseases are highly complex making them challenging to model in cell culture. All cell types of the brain have been implicated as exerting an effect on pathogenesis, and disease progression is likely influenced by the cross-talk between the different cell types. Sophisticated investigation of the cellular level consequences of cross-talk between different cells types requires three-dimensional (3D) co-culture systems.

New method

Murine neural stem cells were differentiated into mixed-neuronal lineage populations in 3D culture. By seeding these differentiated cultures with microglia from adult brain, we have generated a 3D ex-vivo model of murine brain tissue populated with microglia.

Results

Monitoring the infiltration of GFP-expressing mi

SUBMITTER: Carroll JA 

PROVIDER: S-EPMC8217318 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature