Structural and functional characterization of the receptor binding proteins of Escherichia coli O157 phages EP75 and EP335.

ABSTRACT: Bacteriophages (phages) are widely used as biocontrol agents in food and as antibacterial agents for treatment of food production plant surfaces. An important feature of such phages is broad infectivity towards a given pathogenic species. Phages attach to the surfaces of bacterial cells using receptor binding proteins (RBPs), namely tail fibers or tailspikes (TSPs). The binding range of RBPs is the primary determinant of phage host range and infectivity, and therefore dictates a phage's suitability as an antibacterial agent. Phages EP75 and EP335 broadly infect strains of E. coli serotype O157. To better understand host recognition by both phages, here we focused on characterizing the structures and functions of their RBPs. We identified two distinct tail fibers in the genome of the

SUBMITTER: Witte S 

PROVIDER: S-EPMC8217332 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature