Transcriptional and imprinting complexity in Arabidopsis seeds at single-nucleus resolution.

ABSTRACT: Seeds are a key life cycle stage for many plants. Seeds are also the basis of agriculture and the primary source of calories consumed by humans¹. Here, we employ single-nucleus RNA-sequencing to generate a transcriptional atlas of developing Arabidopsis thaliana seeds, with a focus on endosperm. Endosperm, the primary site of gene imprinting in flowering plants, mediates the relationship between the maternal parent and the embryo². We identify transcriptionally uncharacterized nuclei types in the chalazal endosperm, which interfaces with maternal tissue for nutrient unloading^3,4. We demonstrate that the extent of parental bias of maternally expressed imprinted genes varies with cell-cycle phase, and that imprinting of paternally expressed imprinted genes is

SUBMITTER: Picard CL 

PROVIDER: S-EPMC8217372 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature