Fatty acid desaturase activity in mature red blood cells and implications for blood storage quality.

ABSTRACT:

Background

Increases in the red blood cell (RBC) degree of fatty acid desaturation are reported in response to exercise, aging, or diseases associated with systemic oxidant stress. However, no studies have focused on the presence and activity of fatty acid desaturases (FADS) in the mature RBC.

Study design and methods

Steady state metabolomics and isotope-labeled tracing experiments, immunofluorescence approaches, and pharmacological interventions were used to determine the degree of fatty acid unsaturation, FADS activity as a function of storage, oxidant stress, and G6PD deficiency in human and mouse RBCs.

Results

In 250 blood units from the REDS III RBC Omics recalled donor population, we report a storage-dependent accumulation of free mono-, poly-(PUFAs), and highl

SUBMITTER: Thomas T 

PROVIDER: S-EPMC8217387 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature