Systematic truncations of chromosome 4 and their responses to antifungals in Candida albicans.

ABSTRACT:

Background

Candida albicans is an opportunistic human fungal pathogen responsible for superficial and systemic life-threatening infections. Treating these infections is challenging as many clinical isolates show increased drug resistance to antifungals. Chromosome (Chr) 4 monosomy was implicated in a fluconazole-resistant mutant. However, exposure to fluconazole adversely affects Candida cells and can generate numerous mutations. Hence, the present study aimed to truncate Chr4 and challenge the generated Candida strains to antifungals and evaluate their role in drug response.

Results

Herein, Chr4 was truncated in C. albicans using the telomere-mediated chromosomal truncation method. The resulting eight Candida strains carrying one truncated homolog of Chr4 were tested for re

SUBMITTER: Uddin W 

PROVIDER: S-EPMC8217416 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature