High-resolution transcriptional landscape of xeno-free human induced pluripotent stem cell-derived cerebellar organoids.

ABSTRACT: Current protocols for producing cerebellar neurons from human pluripotent stem cells (hPSCs) often rely on animal co-culture and mostly exist as monolayers, limiting their capability to recapitulate the complex processes in the developing cerebellum. Here, we employed a robust method, without the need for mouse co-culture to generate three-dimensional cerebellar organoids from hPSCs that display hallmarks of in vivo cerebellar development. Single-cell profiling followed by comparison to human and mouse cerebellar atlases revealed the presence and maturity of transcriptionally distinct populations encompassing major cerebellar cell types. Encapsulation with Matrigel aimed to provide more physiologically-relevant conditions through recapitulation of basement-membrane signalling, influenced b

SUBMITTER: Nayler S 

PROVIDER: S-EPMC8217544 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature