A design and optimization of a high throughput valve based microfluidic device for single cell compartmentalization and analysis.

ABSTRACT: The need for high throughput single cell screening platforms has been increasing with advancements in genomics and proteomics to identify heterogeneity, unique cell subsets or super mutants from thousands of cells within a population. For real-time monitoring of enzyme kinetics and protein expression profiling, valve-based microfluidics or pneumatic valving that can compartmentalize single cells is advantageous by providing on-demand fluid exchange capability for several steps in assay protocol and on-chip culturing. However, this technique is throughput limited by the number of compartments in the array. Thus, one big challenge lies in increasing the number of microvalves to several thousand that can be actuated in the microfluidic device to confine enzymes and substrates in picoliter vol

SUBMITTER: Briones J 

PROVIDER: S-EPMC8217553 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature