Novel antigen panel for modern broad-spectrum recombinant rotavirus A vaccine.

ABSTRACT:

Purpose

Recombinant rotavirus A vaccines are being developed as an alternative to existing live oral attenuated vaccines. One of the main problems in the production of such vaccines is the genetic diversity of the strains that are in circulation. The goal of this study was to create an antigen panel for modern broad-spectrum recombinant rotavirus A vaccine.

Materials and methods

The antigens of rotavirus were cloned and expressed in Escherichia coli. Antigenic specificity was investigated by Western blot analysis, which was performed using commercial polyclonal antisera to several RVA strains. Phylogenetic analysis was based on the amino acid sequences of the VP8^* protein fragment of human RVA isolates representing genotypes P[4], P[6], and P[8].

Results

SUBMITTER: Kondakova OA 

PROVIDER: S-EPMC8217573 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature