Zeta-carbonic anhydrases show CS₂ hydrolase activity: A new metabolic carbon acquisition pathway in diatoms?

ABSTRACT: CDCA1 is a very peculiar member of the Carbonic Anhydrase (CA) family. It has been the first enzyme to show an efficient utilization of Cd(II) ions in Nature and a unique adaptation capability to live on the surface ocean. Indeed, in this environment, which is extremely depleted in essential metal ions, CDCA1 can utilize Zn(II) or Cd(II) as catalytic metal to support the metabolic needs of fast growing diatoms. In this paper we demonstrate a further catalytic versatility of this enzyme by using a combination of X-ray crystallography, molecular dynamics simulations and enzymatic experiments. First we identified the CO₂ binding site and the way in which this substrate travels from the environment to the enzyme active site. Then, starting from the observation of a structural simila

SUBMITTER: Alterio V 

PROVIDER: S-EPMC8217695 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature