Cell-Free Characterization of Coherent Feed-Forward Loop-Based Synthetic Genetic Circuits.

ABSTRACT: Regulatory pathways inside living cells employ feed-forward architectures to fulfill essential signal processing functions that aid in the interpretation of various types of inputs through noise-filtering, fold-change detection and adaptation. Although it has been demonstrated computationally that a coherent feed-forward loop (CFFL) can function as noise filter, a property essential to decoding complex temporal signals, this motif has not been extensively characterized experimentally or integrated into larger networks. Here we use post-transcriptional regulation to implement and characterize a synthetic CFFL in an Escherichia coli cell-free transcription-translation system and build larger composite feed-forward architectures. We employ microfluidic flow reactors to probe the respon

SUBMITTER: Pieters PA 

PROVIDER: S-EPMC8218305 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature