Identification of juvenile hormone-induced posttranslational modifications of methoprene tolerant and Kruppel homolog 1 in the yellow fever mosquito, Aedes aegypti.

ABSTRACT: Recent studies reported that JH-regulated phosphorylation status of the JH-receptor complex contributes to its transcription activity in Aedes aegypti. However, phosphorylation sites of these proteins have not yet been identified. In this study, we found that the fusion of an EGFP tag to Ae. aegypti Kr-h1 (AaKr-h1) and Met (AaMet) improved their stability in mosquito Aag-2 cells, which allowed their purification. The liquid chromatography and tandem mass spectrometry analysis of the purified AaKr-h1 showed that the phosphoserine residue at position 694, located in the evolutionarily conserved SVIQ motif, is dephosphorylated when the cells are exposed to JH. The AaKr-h1 dephosphorylation mutant (S694V) showed significantly higher activity in inducing the luciferase gene regulated by JH resp

SUBMITTER: Kim K 

PROVIDER: S-EPMC8218339 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature