Protocols for isolating and characterizing polysaccharides from plant cell walls: a case study using rhamnogalacturonan-II.

ABSTRACT:

Background

In plants, a large diversity of polysaccharides comprise the cell wall. Each major type of plant cell wall polysaccharide, including cellulose, hemicellulose, and pectin, has distinct structures and functions that contribute to wall mechanics and influence plant morphogenesis. In recent years, pectin valorization has attracted much attention due to its expanding roles in biomass deconstruction, food and material science, and environmental remediation. However, pectin utilization has been limited by our incomplete knowledge of its structure. Herein, we present a workflow of principles relevant for the characterization of polysaccharide primary structure using nature's most complex polysaccharide, rhamnogalacturonan-II (RG-II), as a model.

Results

We outline how to

SUBMITTER: Barnes WJ 

PROVIDER: S-EPMC8218411 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature