Cannabinoid Receptor Activation on Haematopoietic Cells and Enterocytes Protects against Colitis.

ABSTRACT:

Background and aims

Cannabinoid receptor [CB] activation can attenuate inflammatory bowel disease [IBD] in experimental models and human cohorts. However, the roles of the microbiome, metabolome, and the respective contributions of haematopoietic and non-haematopoietic cells in the anti-colitic effects of cannabinoids have yet to be determined.

Methods

Female C57BL/6 mice were treated with either cannabidiol [CBD], Δ 9-tetrahydrocannabinol [THC], a combination of CBD and THC, or vehicle, in several models of chemically induced colitis. Clinical parameters of colitis were assessed by colonoscopy, histology, flow cytometry, and detection of serum biomarkers; single-cell RNA sequencing and qRT-PCR were used to evaluate the effects of cannabinoids on enterocytes. Immune cell tra

SUBMITTER: Becker W 

PROVIDER: S-EPMC8218712 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature