Missing-in-metastasis protein promotes internalization of magnetic nanoparticles via association with clathrin light chain and Rab7.

ABSTRACT:

Background

Magnetic nanoparticles (MNPs) have been widely used in biomedical applications. Proper control of the duration of MNPs in circulation promises to improve further their applications, in particularly drug delivery. It is known that the uptake of tissue-associated MNPs is mainly carried out by macrophages. Yet, the molecular mechanism to control MNPs internalization in macrophages remains to be elusive. Missing-in-metastasis (MIM) is a scaffolding protein that is highly expressed in macrophages and regulates receptor-mediated endocytosis. We hypothesize that uptake of MNPs may also involve the function of MIM.

Methods

We investigated the effect of MIM expression on the intracellular trafficking of MNPs by transmission electronic microscopy, flow cytometry, o-phenanth

SUBMITTER: Zhao P 

PROVIDER: S-EPMC8218922 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature