Overexpression of thioredoxin m in chloroplasts alters carbon and nitrogen partitioning in tobacco.

ABSTRACT: In plants, there is a complex interaction between carbon (C) and nitrogen (N) metabolism, and its coordination is fundamental for plant growth and development. Here, we studied the influence of thioredoxin (Trx) m on C and N partitioning using tobacco plants overexpressing Trx m from the chloroplast genome. The transgenic plants showed altered metabolism of C (lower leaf starch and soluble sugar accumulation) and N (with higher amounts of amino acids and soluble protein), which pointed to an activation of N metabolism at the expense of carbohydrates. To further delineate the effect of Trx m overexpression, metabolomic and enzymatic analyses were performed on these plants. These results showed an up-regulation of the glutamine synthetase-glutamate synthase pathway; specifically tobacco plan

SUBMITTER: Ancin M 

PROVIDER: S-EPMC8219043 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature