Tracking break-induced replication shows that it stalls at roadblocks.

ABSTRACT: Break-induced replication (BIR) repairs one-ended double-strand breaks in DNA similar to those formed by replication collapse or telomere erosion, and it has been implicated in the initiation of genome instability in cancer and other human diseases^1,2. Previous studies have defined the enzymes that are required for BIR^1-5; however, understanding of initial and extended BIR synthesis, and of how the migrating D-loop proceeds through known replication roadblocks, has been precluded by technical limitations. Here we use a newly developed assay to show that BIR synthesis initiates soon after strand invasion and proceeds more slowly than S-phase replication. Without primase, leading strand synthesis is initiated efficiently, but is unable to proceed beyond 30 kilobases, su

SUBMITTER: Liu L 

PROVIDER: S-EPMC8219245 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature