Locating macromolecular assemblies in cells by 2D template matching with cisTEM.

ABSTRACT: For a more complete understanding of molecular mechanisms, it is important to study macromolecules and their assemblies in the broader context of the cell. This context can be visualized at nanometer resolution in three dimensions (3D) using electron cryo-tomography, which requires tilt series to be recorded and computationally aligned, currently limiting throughput. Additionally, the high-resolution signal preserved in the raw tomograms is currently limited by a number of technical difficulties, leading to an increased false-positive detection rate when using 3D template matching to find molecular complexes in tomograms. We have recently described a 2D template matching approach that addresses these issues by including high-resolution signal preserved in single-tilt images. A current limi

SUBMITTER: Lucas BA 

PROVIDER: S-EPMC8219381 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature