Unexpected contribution of fibroblasts to muscle lineage as a mechanism for limb muscle patterning.

ABSTRACT: Positional information driving limb muscle patterning is contained in connective tissue fibroblasts but not in myogenic cells. Limb muscles originate from somites, while connective tissues originate from lateral plate mesoderm. With cell and genetic lineage tracing we challenge this model and identify an unexpected contribution of lateral plate-derived fibroblasts to the myogenic lineage, preferentially at the myotendinous junction. Analysis of single-cell RNA-sequencing data from whole limbs at successive developmental stages identifies a population displaying a dual muscle and connective tissue signature. BMP signalling is active in this dual population and at the tendon/muscle interface. In vivo and in vitro gain- and loss-of-function experiments show that BMP signalling regulates a fib

SUBMITTER: Esteves de Lima J 

PROVIDER: S-EPMC8219714 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature