Selective inheritance of target genes from only one parent of sexually reproduced F1 progeny in Arabidopsis.

ABSTRACT: Sexual reproduction constrains progeny to inherit allelic genes from both parents. Selective acquisition of target genes from only one parent in the F1 generation of plants has many potential applications including the elimination of undesired alleles and acceleration of trait stacking. CRISPR/Cas9-based gene drives can generate biased transmission of a preferred allele and convert heterozygotes to homozygotes in insects and mice, but similar strategies have not been implementable in plants because of a lack of efficient homology-directed repair (HDR). Here, we place a gene drive, which consists of cassettes that produce Cas9, guide RNAs (gRNA), and fluorescent markers, into the CRYPTOCHROME 1 (CRY1) gene through CRISPR/Cas9-mediated HDR, resulting in cry1^drive lines. After cros

SUBMITTER: Zhang T 

PROVIDER: S-EPMC8219824 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature