Protocol to analyze circulating small non-coding RNAs by high-throughput RNA sequencing from human plasma samples.
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ABSTRACT:
SUBMITTER: Grieco GE
PROVIDER: S-EPMC8219884 | biostudies-literature | 2021 Sep
REPOSITORIES: biostudies-literature
Publications
Protocol to analyze circulating small non-coding RNAs by high-throughput RNA sequencing from human plasma samples.
Grieco Giuseppina E GE Sebastiani Guido G Fignani Daniela D Brusco Noemi N Nigi Laura L Formichi Caterina C Licata Giada G Bruttini Marco M D'Aurizio Romina R Mathieu Chantal C Gysemans Conny C Dotta Francesco F
STAR protocols 20210615 3
The identification and validation of circulating small non-coding RNA (sncRNA) as biomarkers for disease diagnosis, staging, and response to novel therapies is still a compelling challenge. Pre-analytical variables, such as storage temperature or blood hemolysis, and different analytical approaches affect sncRNA stability, detection, and expression, resulting in discrepancies among studies. Here, we report a systematic standardized protocol to reproducibly analyze circulating sncRNAs, employing ...[more]
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