Real-time identification of two substrate-binding intermediates for the light-driven sodium pump rhodopsin.

ABSTRACT: Membrane transport proteins undergo critical conformational changes during substrate uptake and release, as the substrate-binding site is believed to switch its accessibility from one side of the membrane to the other. Thus, at least two substrate-binding intermediates should appear during the process, that is, after uptake and before the release of the substrate. However, this view has not been verified for most transporters because of the difficulty in detecting short-lived intermediates. Here, we report real-time identification of these intermediates for the light-driven outward current-generating Na⁺-pump rhodopsin. We triggered the transport cycle of Na⁺-pump rhodopsin using a short laser pulse, and subsequent formation and decay of various intermediates was dete

SUBMITTER: Kato T 

PROVIDER: S-EPMC8219890 | biostudies-literature | 2021 Jan-Jun

REPOSITORIES: biostudies-literature