PRISMA and BioID disclose a motifs-based interactome of the intrinsically disordered transcription factor C/EBPα.

ABSTRACT: C/EBPα represents a paradigm intrinsically disordered transcription factor containing short linear motifs and post-translational modifications (PTM). Unraveling C/EBPα protein interaction networks is a prerequisite for understanding the multi-modal functions of C/EBPα in hematopoiesis and leukemia. Here, we combined arrayed peptide matrix screening (PRISMA) with BioID to generate an in vivo validated and isoform specific interaction map of C/EBPα. The myeloid C/EBPα interactome comprises promiscuous and PTM-regulated interactions with protein machineries involved in gene expression, epigenetics, genome organization, DNA replication, RNA processing, and nuclear transport. C/EBPα interaction hotspots coincide with homologous conserved regions of the C/EBP family that also score as mol

SUBMITTER: Ramberger E 

PROVIDER: S-EPMC8220391 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature