Detection of cell-free microbial DNA using a contaminant-controlled analysis framework.

ABSTRACT:

Background

The human microbiome plays an important role in cancer. Accumulating evidence indicates that commensal microbiome-derived DNA may be represented in minute quantities in the cell-free DNA of human blood and could possibly be harnessed as a new cancer biomarker. However, there has been limited use of rigorous experimental controls to account for contamination, which invariably affects low-biomass microbiome studies.

Results

We apply a combination of 16S-rRNA-gene sequencing and droplet digital PCR to determine if the specific detection of cell-free microbial DNA (cfmDNA) is possible in metastatic melanoma patients. Compared to matched stool and saliva samples, the absolute concentration of cfmDNA is low but significantly above the levels detected from negative contr

SUBMITTER: Zozaya-Valdes E 

PROVIDER: S-EPMC8220693 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature