New homozygous gpt delta transgenic rat strain improves an efficiency of the in vivo mutagenicity assay.

ABSTRACT:

Background

Gene mutation assays in transgenic rodents are useful tools to investigate in vivo mutagenicity in a target tissue. Using a lambda EG10 transgene containing reporter genes, gpt delta transgenic mice and rats have been developed to detect point mutations and deletions. The transgene is integrated in the genome and can be rescued through an in vitro packaging reaction. However, the packaging efficiency is lower in gpt delta rats than in mice, because of the transgene in gpt delta rats being heterozygous and in low copy number. To improve the packaging efficiency, we herein describe a newly developed homozygous gpt delta rat strain.

Results

The new gpt delta rat has a Wistar Hannover background and has been successfully maintained as homozygous for the transgene. The

SUBMITTER: Masumura K 

PROVIDER: S-EPMC8220708 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature