Long-term culture, genetic manipulation and xenotransplantation of human normal and breast cancer organoids.

ABSTRACT: Organoid technology has revolutionized the study of human organ development, disease and therapy response tailored to the individual. Although detailed protocols are available for the generation and long-term propagation of human organoids from various organs, such methods are lacking for breast tissue. Here we provide an optimized, highly versatile protocol for long-term culture of organoids derived from either normal human breast tissues or breast cancer (BC) tissues, as well as culturing conditions for a panel of 45 biobanked samples, including BC organoids covering all major disease subtypes (triple-negative, estrogen receptor-positive/progesterone receptor-positive and human epidermal growth receptor 2-positive). Additionally, we provide methods for genetic manipulation by Lipofectami

SUBMITTER: Dekkers JF 

PROVIDER: S-EPMC8221035 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature