Fast-responding functional DNA superstructures for stimuli-triggered protein release.

ABSTRACT: Strategies that speed up the on-command release of proteins (e.g., enzymes) from stimuli-responsive materials are intrinsically necessary for biosensing applications, such as point-of-care testing, as they will achieve fast readouts with catalytic signal-amplification. However, current systems are challenging to work with because they usually exhibit response times on the order of hours up to days. Herein, we report on the first effort to construct a fast-responding gating system using protein-encapsulating functional DNA superstructures (denoted as protein@3D DNA). Proteins were directly embedded into 3D DNA during the one-pot rolling circle amplification process. We found that the specific DNA-DNA interaction and aptamer-ligand interaction could act as general protocols to release

SUBMITTER: Zhang Y 

PROVIDER: S-EPMC8221054 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature