Age-related defects in autophagy alter the secretion of paracrine factors from bone marrow mononuclear cells.

ABSTRACT: Bone marrow mononuclear cell therapy improves cardiac repair after myocardial infarction (MI), in-part through signaling to resident cardiac cells, such as fibroblasts, which regulate scar formation. The efficacy of cell therapy declines with age, as aging of both donor and recipient cells decreases repair responses. Autophagy regulates the microenvironment by both extracellular vesicle (EV)-dependent and independent secretion pathways. We hypothesized that age-related autophagy changes in bone marrow cells (BMCs) alter paracrine signaling, contributing to lower cell therapy efficacy. Here, we demonstrate that young Sca-1⁺ BMCs exhibited a higher LC3II/LC3I ratio compared to old Sca-1⁺ BMCs, which was accentuated when BMCs were cultured under hypoxia. To examine the e

SUBMITTER: Yeganeh A 

PROVIDER: S-EPMC8221303 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature