Integrated multi-wavelength microscope combining TIRFM and IRM modalities for imaging cellulases and other processive enzymes.
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ABSTRACT:
SUBMITTER: Nong D
PROVIDER: S-EPMC8221963 | biostudies-literature | 2021 Jun
REPOSITORIES: biostudies-literature
Publications
Integrated multi-wavelength microscope combining TIRFM and IRM modalities for imaging cellulases and other processive enzymes.
Nong Daguan D Haviland Zachary K ZK Kuntz Kate Vasquez KV Tien Ming M Anderson Charles T CT Hancock William O WO
Biomedical optics express 20210511 6
We describe a multimodal microscope for visualizing processive enzymes moving on immobilized substrates. The instrument combines interference reflection microscopy (IRM) with multi-wavelength total internal reflectance fluorescence microscopy (TIRFM). The microscope can localize quantum dots with a precision of 2.8 nm at 100 frames/s, and was used to image the dynamics of the cellulase, Cel7a interacting with surface-immobilized cellulose. The instrument, which was built with off-the-shelf compo ...[more]
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