Single-objective high-resolution confocal light sheet fluorescence microscopy for standard biological sample geometries.

ABSTRACT: Three-dimensional fluorescence-based imaging of living cells and organisms requires the sample to be exposed to substantial excitation illumination energy, typically causing phototoxicity and photobleaching. Light sheet fluorescence microscopy dramatically reduces phototoxicity, yet most implementations are limited to objective lenses with low numerical aperture and particular sample geometries that are built for specific biological systems. To overcome these limitations, we developed a single-objective light sheet fluorescence system for biological imaging based on axial plane optical microscopy and digital confocal slit detection, using either Bessel or Gaussian beam shapes. Compared to spinning disk confocal microscopy, this system displays similar optical resolution, but a significantl

SUBMITTER: Yordanov S 

PROVIDER: S-EPMC8221969 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature